LOCUS       sequence-71733-e        2332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For inserting selection gene between AttR2 and AttL3 sites for use 
            in Gateway recombination cloning system (corresponds to a module 3 
            plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-L3    #BGV193
FEATURES             Location/Qualifiers
     source          1..2332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    102..123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     183..205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     202..218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(249..373)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(450..545)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(574..593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(575..593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(600..617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(600..616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(823..1641)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSSRPRLNSNMDADLYGYKWARDNVGQSGATIY*LY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1546..1565
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1633..1652
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1758..2302
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2203..2222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
       61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      121 cattaggcac cccaggcttt acactttatg cttccgcggc tcgtatgttg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc actagcagat
      241 ctgccggcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
      301 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
      361 tgtacaaagt ggtgctagcg cggccgcacg cgtgctgagg agacatctag actttccctc
      421 agcgtcgacg atatcggcgc gcccccgggc aactttatta tacaaagttg gcattataaa
      481 aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      541 tatttactag tgttaacttc gatccccaat tcgccctata gtgagtcgta ttacaattca
      601 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      661 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      721 ccttcccaac agttgcgcag cctgaatggc gaatgagctt gcgccgtccc gtcaagtcag
      781 cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
      841 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
      901 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
      961 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     1021 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     1081 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     1141 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     1201 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     1261 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     1321 tgctgttttc ccagggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     1381 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     1441 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     1501 cttcccatac aatcaataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     1561 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg agcaagacgt
     1621 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     1681 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     1741 tcgacaagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     1801 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
     1861 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     1921 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
     1981 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2041 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     2101 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     2161 accgaaaggc aggaacagga gagcgcacga gggagccgcc aggggaaacg cctggtatct
     2221 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     2281 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cc
//
