LOCUS       sequence-71737-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 StrepII TEV co-transformation cloning...
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    148..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             224..246
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             266..283
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             299..322
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             329..349
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(474..492)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      488..535
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagagaa ttgtgagcgg ataacaattc ccctgtagat
      181 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      241 aggagatata catatgaaat cttctcacca tcaccatcac catggttctt ctgctagctg
      301 gagccacccg cagttcgaaa aaggcgccga aaacctgtac ttccaatcca atattggaag
      361 tggataacgg atccgcgatc gcggcgcgcc acctggtggc cggccggtac cacgcgtgcg
      421 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      481 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      541 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
      601 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc
      661 gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac
      721 cttggtgatc tcgcctttca cgtaatggac aaattcttcc aactgatctg cgcgcgaggc
      781 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
      841 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
      901 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
      961 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     1021 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     1081 tcttgctttt gtcagcaag
//
