LOCUS       sequence-71739-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Gateway destination vector constructed by inserting 
            Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway
            Vector Conversion System (Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Shindoh N, Yoda A, Yoda Y, Sullivan TJ, Weigert O, Lane AA, Kopp N, 
            Bird L, Rodig SJ, Fox EA, Weinstock DM
  TITLE     Next-generation cDNA screening for oncogene and resistance 
            phenotypes.
  JOURNAL   PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub
            2012 Nov 7.
  PUBMED    23145123
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49201. doi"; pages: " 
            10.1371/journal.pone.0049201. Epub 2012 Nov 7"
COMMENT     Sequence Label: pMSCVpuroATT
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..166
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(191..196,197..214,215..221)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(341..360)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctccttctct aggcgccgga attagatctc tcgaggttat cacaagtttg tacaaaaaag
       61 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
      121 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
      181 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
      241 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      301 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      361 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      421 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      481 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      541 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
      601 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
      661 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
      721 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
      781 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
      841 tcagg
//
