LOCUS       sequence-71747-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Sumo TEV co-transformation cloning ve...
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             310..603
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             610..630
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(755..773)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      769..816
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagacca caacggtttc cctctagtgc cggctccgga
      181 gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct
      301 atggctagca tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      361 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      421 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      481 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
      541 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
      601 gggatcgagg aaaacctgta cttccaatcc aatattggaa gtggataacg gatccgcgat
      661 cgcggcgcgc cacctggtgg ccggccggta ccacgcgtgc gcgctgatcc ggctgctaac
      721 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      781 cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag
      841 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      901 gctatttaac gaccctgccc tgaaccgacg acaagctgac gaccgggtct ccgcaagtgg
      961 cacttttcgg ggaaatgtgc gcggaacccc
//
