LOCUS       sequence-71750-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 TEV co-transformation cloning vector
            ...
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    148..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             224..246
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             266..283
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             284..304
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(429..447)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      443..490
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagagaa ttgtgagcgg ataacaattc ccctgtagat
      181 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      241 aggagatata catatgaaat cttctcacca tcaccatcac catgaaaacc tgtacttcca
      301 atccaatatt ggaagtggat aacggatccg cgatcgcggc gcgccacctg gtggccggcc
      361 ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      421 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      481 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
      541 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
      601 gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca
      661 ttatttgccg actaccttgg tgatctcgcc tttcacgtaa tggacaaatt cttccaactg
      721 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      781 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      841 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      901 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      961 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacc
//
