LOCUS       sequence-71754-e        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Ura S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..124
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             171..188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..218
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
       61 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
      121 cgtcaaggag aaaaaacccc ggatccgtaa ccatgtcaac tggttcttct caccatcacc
      181 atcaccatgg ttcttctgaa aacctgtact tccaatccaa tattggaagt ggataactct
      241 cgaggatcgt aatgactaga ttatataatc agaaatagcc tttggtgatc acttccattt
      301 tagtcagcat aatagaaagt ttaattacgc gcttttttac attccgacaa agatctgccc
      361 gagaaaattc ttcgagaaga caacaacaac aaaaaaaata aagcctgggc gaaaaacatc
      421 gtgcgtttac ataatatctt gaaaagcaaa cagatatata aaccgctatt gaaattacct
      481 ttttaagtat tcgcctagaa gccttgtttc aagtagcatc aacaacaggt gttaggttaa
      541 actgctttga ctttttgttc tttaacgaat atcattaaaa aaaaaggaca tctataaaga
      601 atgtctccaa actcgtcgaa aactcgaacg gaccagataa gctccatgcc tggtattaat
      661 gaagccacga aggtggaaag taagaatgtt gtgaaggatg ctgttccaat aaaatctgaa
      721 gttgaaacca atggtacatc aatagtaaga gaaaaacaag atccacagga cgggtgtggt
      781 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
      841 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
      901 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
      961 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     1021 gaggatgacg atgagcgcat tgttagattt catacacggt gc
//
