LOCUS       sequence-71757-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Ade S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..126
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             173..190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta
       61 cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt
      121 aacgtcaagg agaaaaaacc ccggatccgt aaccatgtca actggttctt ctcaccatca
      181 ccatcaccat ggttcttctg aaaacctgta cttccaatcc aatattggaa gtggataact
      241 ctcgaggatc gtaatgacta gattatataa tcagaaatag cctttggtga tcacttccat
      301 tttagtcagc ataatagaaa gtttaattac gcgctttttt acattccgac aaagatctgc
      361 ccgagaaaat tcttcgagaa gacaacaaca acaaaaaaaa taaagcctgg gcgaaaaaca
      421 tcgtgcgttt acataatatc ttgaaaagca aacagatata taaaccgcta ttgaaattac
      481 ctttttaagt attcgcctag aagccttgtt tcaagtagca tcaacaacag gtgttaggtt
      541 aaactgcttt gactttttgt tctttaacga atatcattaa aaaaaaagga catctataaa
      601 gaatgtctcc aaactcgtcg aaaactcgaa cggaccagat aagctccatg cctggtatta
      661 atgaagccac gaaggtggaa agtaagaatg ttgtgaagga tgctgttcca ataaaatctg
      721 aagttgaaac caatggtaca tcaatagtaa gagaaaaaca agatccacag gacgggtgtg
      781 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
      841 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
      901 tatagcgcta gcagcacgcc atagtgactg gtgatgctgt cggaatggac gatatcccgc
      961 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
     1021 ccgaggatga cgatgagcgc attgttagat t
//
