LOCUS       sequence-71760-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Ade S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1050..1070)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1080..1097)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgct ctagaactag tggttccccc gggaatctcg gtcgtaatga
      121 tttttataat gacgaaaaaa aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc
      181 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
      241 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
      301 gggcctcttg cgggatatcg tccattccga cagcatcacc agtcactatg gcgtgctgct
      361 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
      421 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
      481 catggcgacc acacccgtcc tgtggatctt gtttttctct tactattgat gtaccattgg
      541 tttcaacttc agattttatt ggaacagcat ccttcacaac attcttactt tccaccttcg
      601 tggcttcatt aataccaggc atggagctta tctggtccgt tcgagttttc gacgagtttg
      661 gagacattct ttatagatgt cctttttttt taatgatatt cgttaaagaa caaaaagtca
      721 aagcagttta acctaacacc tgttgttgat gctacttgaa acaaggcttc taggcgaata
      781 cttaaaaagg taatttcaat agcggtttat atatctgttt gcttttcaag atattatgta
      841 aacgcacgat gtttttcgcc caggctttat tttttttgtt gttgttgtct tctcgaagaa
      901 ttttctcggg cagatctttg tcggaatgta aaaaagcgcg taattaaact ttctattatg
      961 ctgactaaaa tggaagtgat caccaaaggc tatttctgat tatataatct agtcattacg
     1021 atcctcgaga gttatccact tccaatattg gattggaagt acaggttttc agaagaacca
     1081 tggtgatggt gatggtgaga agaaccagtt gacatggtta cggatccggg gtttt
//
