LOCUS       sequence-71765-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 Trp S. cerevisiae expression vector
            (1...
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        93..240
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             241..915
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 acagcatcgt aatatatgtg tactttgcag ttatgacgcc agatggcagt agtggaagat
       61 attctttatt gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga gcttttcttt
      121 ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg
      181 cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt
      241 atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag
      301 agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt
      361 gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta
      421 aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa
      481 cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat
      541 ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc
      601 gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt
      661 attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt
      721 tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga
      781 ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta
      841 agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa
      901 aatgctaaga aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc
      961 cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     1021 ttgtaaacgt taataatttg
//
