LOCUS       sequence-71770-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6-MBP TEV Ade S. cerevisiae express...
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..40)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    413..460
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
       61 taataatggt ttcttagtat gatccaatat caaaggaaat gatagcattg aaggatgaga
      121 ctaatccaat tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca
      181 tacgataccc cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct
      241 acataaatag acgcatataa gtacgcattt aagcataaac acgcactatg ccgttcttct
      301 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
      361 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
      421 tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt tgaaaaccaa
      481 aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact
      541 aaaataatgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc
      601 tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta
      661 aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg
      721 tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata
      781 cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc
      841 atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa
      901 tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc
      961 gggaagtgga gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc
     1021 taaccttaac ggacctac
//
