LOCUS       sequence-71783-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Kessels HW, Kopec CD, Klein ME, Malinow R
  TITLE     Roles of stargazin and phosphorylation in the control of AMPA 
            receptor subcellular distribution.
  JOURNAL   Nat Neurosci. 2009 Jul . 12(7):888-96.
  PUBMED    19543281
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2009 Jul . 12(7):888-96."
COMMENT     Sequence Label: pSIN ReP5-GFP-GluR1
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..127
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(258..260,261..773)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(300..321)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(561..580)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggatcccccg ggctgcagga attcggcacg agctcggctc cccttccaag agaaacaaga
       61 gaaacctcac ggaaggaagg gaggaaggaa agaagccggc agagcatcaa gaagaatcga
      121 agggagggga gggaagacca aatctatggt tggaccaggg cttctttttc gccaatgtaa
      181 aaaggaatat gccgtacatc tttgcctttt tctgcaccgg ttttctaggt gcggttgtgg
      241 gtgccaattt cgctagggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      301 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      361 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      421 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      481 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      541 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      601 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      661 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      721 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      781 gcgtgcagct cg
//
