LOCUS       sequence-7179-ex        4866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, 
            Bartel DP
  TITLE     The widespread impact of mammalian MicroRNAs on mRNA repression and 
            evolution.
  JOURNAL   Science. 2005 Dec 16. 310(5755):1817-21.
  PUBMED    16308420
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Dec 16. 310(5755):1817-21."
COMMENT     Sequence Label: pAG70 BDNF 3'UTR
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1181..1302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1192..1211)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1246..1265
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1435..1539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1540..1608,1609..2400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1758..1777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2571..3159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3060..3079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3288..3645
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3288..3479
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3311..3331)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3496..3631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3715..3847
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3830..3849
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3892..3910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3920..4855
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 ccagtcattt gcgcacaact taaaaagtct gcattacatt ccttgataat gttgtggttt
       61 gttgccgttg ccaagaactg aaaacataaa aagttaaaaa aaataataaa ttgcatgctg
      121 ctttaattgt gaattgataa taaactgtcc tctttcagaa aacagaaaaa aaacacacac
      181 acacacaaca aaaatttgaa ccaaaacatt ccgtttacat tttagacagt aagtatcttc
      241 gttcttgtta gtactatatc tgttttactg cttttaactt ctgatagcgt tggaattaaa
      301 acaatgtcaa ggtgctgttg tcattgcttt actggcttag gggatggggg atggggggta
      361 tatttttgtt tgttttgtgt ttttttttcg tttgtttgtt ttgtttttta gttcccacag
      421 ggagtagaga tggggaaaga attcctacaa tatatattct ggctgataaa agatacattt
      481 gtatgttgtg aagatgtttg caatatcgat cagatgacta gaaagtgaat aaaaattaag
      541 gcaactgaac aaaaaaatgc tcacactcca catcccgtga tgcacctccc aggccccgct
      601 cattctttgg gcgttggtca gagtaagctg cttttgacgg aaggacctat gtttgctcag
      661 aacacattct ttccccccct ccccctctgg tctcctcttt gttttgtttt aaggaagaaa
      721 aatcagttgc gcgttctgaa atattttacc actgctgtga acaagtgaac acattgtgtc
      781 acatcatgac actcgtataa gcatggagaa cagtgatttt tttttagaac agaaaacaac
      841 aaaaaataac cccaaaatga agattatttt ttatgaggag tgaacatttg ggtaaatcat
      901 ggctaagctt aaaaaaaact catggtgagg cttaacaatg tcttgtaagc aaaaggtaga
      961 gccctgtatc aacccagaaa cacctagatc agaacaggaa tccacattgc cagtgacatg
     1021 agactgaaca gccaaatgga ggctatgtgg agttggcatt gcatttaccg gcagtgcggg
     1081 aggaatttct gagtggccat cccaaggtct aggtggaggt ggggcatggt atttgagaca
     1141 ttccaaaacg atctagagcg gccgcttcga gcagacatga taagatacat tgatgagttt
     1201 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1261 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1321 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     1381 tacaaatgtg gtaaaatcga taaggatcca ggtggcactt ttcggggaaa tgtgcgcgga
     1441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1501 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1561 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1621 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1681 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1741 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1801 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1861 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1921 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1981 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2041 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2101 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2161 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2221 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2281 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2341 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3181 ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat ctgcgcagca
     3241 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     3301 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct
     3661 gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt
     3721 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga
     3781 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc
     3841 tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac
     3901 tcactatagg ctagccacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat
     3961 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat
     4021 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc
     4081 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat
     4141 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt
     4201 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat
     4261 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca
     4321 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga
     4381 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat
     4441 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt
     4501 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg
     4561 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt
     4621 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt
     4681 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc
     4741 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
     4801 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta
     4861 ggagct
//
