LOCUS       sequence-71819-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene expression vector. Chloramphenicol cm + 
            ccdB cassette may be removed to clone in gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER21 (ORF-EG)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..209
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(234..239,240..257,258..264)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(384..403)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga actagtccag
       61 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
      121 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
      181 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
      301 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      361 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      421 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      481 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
      541 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      601 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
      661 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
      721 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
      781 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
      841 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
      901 tttgtgatgg
//
