LOCUS       sequence-71822-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene expression vector. Chloramphenicol cm + 
            ccdB cassette may be removed to clone in gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER21 (ORF-EG)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(119..736,737..742,743..778,779..781,782..817,818..820,
                     821..856,857..865)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gtgtcgtgaa gcggccgcca tgctagtaaa ttgtccgcta aattctggcc gtttttggct
       61 tttttgttag acggatccat aacttcgtat aatgtatgct atacgaagtt atgccaccat
      121 gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
      181 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
      241 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
      301 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
      361 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
      421 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
      481 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
      541 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
      601 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
      661 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
      721 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
      781 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
      841 tgaccttgac atgctccccg ggtaactaag taaggatccg cggccgcact aga
//
