LOCUS       sequence-71828-e        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..754)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(755..808)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgactctag aatcaattaa
       61 gcttgtgccc cagtttgcta gggaggtcgc agtatctggc cactgccacc tcgtgctgct
      121 cgacgtaggt ctcgttgttg gcctccttga ttctttccag tctgtagtcc acatagtaga
      181 cgccaggcat cttgaggttc ttagcgggtt tcttggatct atatgtggtc ttgatgtttg
      241 cgatcagatg gctcccaccc acgagcttca gggccatgtc gtttctgcct tccaggccgc
      301 cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc gagtgttttc ttctgcatca
      361 cagggccgtt ggatgtgaag ttcacccctc tgatcttgac gttgtagatg aggcagccgt
      421 cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat gtggtgactc
      481 tctcccatgt gaagccctca gggaaggact gcttgaagaa gtcggggatg ccctgggtgt
      541 ggttgatgaa ggtcttgctg ccgtagagga agctagtagc caggatgtcg aaggcgaagg
      601 ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg tagggcttgc
      661 cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac ggtgccctcc atgtacagct
      721 tcatgtgcat gttctcctta atcagctcgc tcatggggcc cggattctcc tccacgtcac
      781 cgcatgttag aagacttcct ctgccctcac cggatccgcc gccgcccttc aggaaggtct
      841 cgctgctgat ggtgttgggc agcttgtaca tcatctgggg gatcaggtag ggcttgatca
      901 ggttgtagaa gatcaggtag ctcatgctgt cgatgtagat gatgggcttg ttcttgttga
      961 tcttcacgta gcagttcagc tggaacttgt tcctcaggcc cttcaccagg tactccacct
     1021 cctcgaaggt gaagctctgg gtgttcagca cgatgctctt gttggtgctg ttcttgttgt
     1081 agtcccactt gccgccgtcg tc
//
