LOCUS       sequence-71829-e        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease in mammalian cells with BFP tracker in 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             176..877
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
       61 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
      121 ttttcctttg aaaaacacga tgataagctt gccacaaccc ttaccggtcg ccaccatgag
      181 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
      241 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      301 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
      361 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
      421 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      481 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      541 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      601 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
      661 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
      721 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
      781 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
      841 cgacctccct agcaaactgg ggcacaagct taattgattc tagagtcgac cgagcatctt
      901 accgccattt atacccatat ttgttctgtt tttcttgatt tgggtataca tttaaatgtt
      961 aatagaacaa aatggtgggg caatcattta catttttagg gatatgtaat tactagttca
     1021 ggtgtattgc cacaagacaa acatgttaag aaactttccc gttatttacg ctctgttcct
     1081 gttaatcaac ctctggatta caaaatttgt gaaagattg
//
