LOCUS       sequence-71849-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Piatkevich KD, Malashkevich VN, Morozova KS, Nemkovich NA, Almo SC, 
            Verkhusha VV
  TITLE     Extended Stokes shift in fluorescent proteins: chromophore-protein 
            interactions in a near-infrared TagRFP675 variant.
  JOURNAL   Sci Rep. 2013;3:1847. doi: 10.1038/srep01847.
  PUBMED    23677204
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1847. doi"; pages: " 10.1038/srep01847"
COMMENT     Sequence Label: pActinin-TagRFP675-N1
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        91..195
                     /gene="bla"
                     /label=AmpR promoter
     promoter        197..554
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        197..388
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(220..240)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      405..540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(643..662)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      121 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      181 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
      241 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      301 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      361 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      421 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      481 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      541 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      601 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      661 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      721 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
      781 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      841 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      901 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      961 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     1021 cgtactcgga tggaagccgg tcttgtc
//
