LOCUS       sequence-71851-e        8881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian NM-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMn-VP64
FEATURES             Location/Qualifiers
     source          1..8881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(748..792,793..795,796..2505,2506..2511,2512..2577,
                     2578..2580,2581..3993)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Neisseria meningitidis Type II 
                     CRISPR/Cas system"
                     /label=NmdCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D16A, D587A, 
                     H588A, and N611A"
                     /translation="MAAFKPNPINYILGLAIGIASVGWAMVEIDEDENPICLIDLGVRV
                     FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIK
                     SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
                     DNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEF
                     GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
                     KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
                     NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
                     KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
                     EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
                     EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
                     RLNEKGYVEIAAALPFSRTWDDSFNNKVLVLGSEAQNKGNQTPYEYFNGKDNSREWQEF
                     KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
                     VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
                     DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKLRTLLAE
                     KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
                     LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
                     TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
                     LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
                     GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     regulatory      3580..3589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4006..4026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4051..4200
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(4267..4286)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4311..4359
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4561..4989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4648..4667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4858..4879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4998..5018)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5003..5332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5183..5318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5245..5264
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5399..6193
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5453..5472)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6063..6082
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6369..6490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6406..6425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6460..6479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6539..6555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6539..6555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6552..6574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6563..6579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6587..6593,6594..6611,6612..6617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6632..6653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6770..6787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6941..7529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7021..7040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7700..8491,8492..8560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8323..8342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8561..8665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8740..8759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagg atcgaaccct tgccaccatg gccgccttca agcccaaccc catcaactac
      781 atcctgggcc tggccatcgg catcgccagc gtgggctggg ccatggtgga gatcgacgag
      841 gacgagaacc ccatctgcct gatcgacctg ggtgtgcgcg tgttcgagcg cgctgaggtg
      901 cccaagactg gtgacagtct ggctatggct cgccggcttg ctcgctctgt tcggcgcctt
      961 actcgccggc gcgctcaccg ccttctgcgc gctcgccgcc tgctgaagcg cgagggtgtg
     1021 ctgcaggctg ccgacttcga cgagaacggc ctgatcaaga gcctgcccaa cactccttgg
     1081 cagctgcgcg ctgccgctct ggaccgcaag ctgactcctc tggagtggag cgccgtgctg
     1141 ctgcacctga tcaagcaccg cggctacctg agccagcgca agaacgaggg cgagaccgcc
     1201 gacaaggagc tgggtgctct gctgaagggc gtggccgaca acgcccacgc cctgcagact
     1261 ggtgacttcc gcactcctgc tgagctggcc ctgaacaagt tcgagaagga gagcggccac
     1321 atccgcaacc agcgcggcga ctacagccac accttcagcc gcaaggacct gcaggccgag
     1381 ctgatcctgc tgttcgagaa gcagaaggag ttcggcaacc cccacgtgag cggcggcctg
     1441 aaggagggca tcgagaccct gctgatgacc cagcgccccg ccctgagcgg cgacgccgtg
     1501 cagaagatgc tgggccactg caccttcgag ccagccgagc ccaaggccgc caagaacacc
     1561 tacaccgccg agcgcttcat ctggctgacc aagctgaaca acctgcgcat cctggagcag
     1621 ggcagcgagc gccccctgac cgacaccgag cgcgccaccc tgatggacga gccctaccgc
     1681 aagagcaagc tgacctacgc ccaggcccgc aagctgctgg gtctggagga caccgccttc
     1741 ttcaagggcc tgcgctacgg caaggacaac gccgaggcca gcaccctgat ggagatgaag
     1801 gcctaccacg ccatcagccg cgccctggag aaggagggcc tgaaggacaa gaagagtcct
     1861 ctgaacctga gccccgagct gcaggacgag atcggcaccg ccttcagcct gttcaagacc
     1921 gacgaggaca tcaccggccg cctgaaggac cgcatccagc ccgagatcct ggaggccctg
     1981 ctgaagcaca tcagcttcga caagttcgtg cagatcagcc tgaaggccct gcgccgcatc
     2041 gtgcccctga tggagcaggg caagcgctac gacgaggcct gcgccgagat ctacggcgac
     2101 cactacggca agaagaacac cgaggagaag atctacctgc ctcctatccc cgccgacgag
     2161 atccgcaacc ccgtggtgct gcgcgccctg agccaggccc gcaaggtgat caacggcgtg
     2221 gtgcgccgct acggcagccc cgcccgcatc cacatcgaga ccgcccgcga ggtgggcaag
     2281 agcttcaagg accgcaagga gatcgagaag cgccaggagg agaaccgcaa ggaccgcgag
     2341 aaggccgccg ccaagttccg cgagtacttc cccaacttcg tgggcgagcc caagagcaag
     2401 gacatcctga agctgcgcct gtacgagcag cagcacggca agtgcctgta cagcggcaag
     2461 gagatcaacc tgggccgcct gaacgagaag ggctacgtgg agatcgccgc tgccctgccc
     2521 ttcagccgca cctgggacga cagcttcaac aacaaggtgc tggtgctggg cagcgaggct
     2581 cagaacaagg gcaaccagac cccctacgag tacttcaacg gcaaggacaa cagccgcgag
     2641 tggcaggagt tcaaggcccg cgtggagacc agccgcttcc cccgcagcaa gaagcagcgc
     2701 atcctgctgc agaagttcga cgaggacggc ttcaaggagc gcaacctgaa cgacacccgc
     2761 tacgtgaacc gcttcctgtg ccagttcgtg gccgaccgca tgcgcctgac cggcaagggc
     2821 aagaagcgcg tgttcgccag caacggccag atcaccaacc tgctgcgcgg cttctggggc
     2881 ctgcgcaagg tgcgcgccga gaacgaccgc caccacgccc tggacgccgt ggtggtggcc
     2941 tgcagcaccg tggccatgca gcagaagatc acccgcttcg tgcgctacaa ggagatgaac
     3001 gccttcgacg gtaaaaccat cgacaaggag accggcgagg tgctgcacca gaagacccac
     3061 ttcccccagc cctgggagtt cttcgcccag gaggtgatga tccgcgtgtt cggcaagccc
     3121 gacggcaagc ccgagttcga ggaggccgac acccccgaga agctgcgcac cctgctggcc
     3181 gagaagctga gcagccgccc tgaggccgtg cacgagtacg tgactcctct gttcgtgagc
     3241 cgcgccccca accgcaagat gagcggtcag ggtcacatgg agaccgtgaa gagcgccaag
     3301 cgcctggacg agggcgtgag cgtgctgcgc gtgcccctga cccagctgaa gctgaaggac
     3361 ctggagaaga tggtgaaccg cgagcgcgag cccaagctgt acgaggccct gaaggcccgc
     3421 ctggaggccc acaaggacga ccccgccaag gccttcgccg agcccttcta caagtacgac
     3481 aaggccggca accgcaccca gcaggtgaag gccgtgcgcg tggagcaggt gcagaagacc
     3541 ggcgtgtggg tgcgcaacca caacggcatc gccgacaacg ccaccatggt gcgcgtggac
     3601 gtgttcgaga agggcgacaa gtactacctg gtgcccatct acagctggca ggtggccaag
     3661 ggcatcctgc ccgaccgcgc cgtggtgcag ggcaaggacg aggaggactg gcagctgatc
     3721 gacgacagct tcaacttcaa gttcagcctg caccccaacg acctggtgga ggtgatcacc
     3781 aagaaggccc gcatgttcgg ctacttcgcc agctgccacc gcggcaccgg caacatcaac
     3841 atccgcatcc acgacctgga ccacaagatc ggcaagaacg gcatcctgga gggcatcggc
     3901 gtgaagaccg ccctgagctt ccagaagtac cagatcgacg agctgggcaa ggagatccgc
     3961 ccctgccgcc tgaagaagcg ccctcctgtg cgcagcaggg ctgaccccaa gaagaagagg
     4021 aaggtggagg ccagcggttc cggacgggct gacgcattgg acgattttga tctggatatg
     4081 ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat
     4141 gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg
     4201 attaactcta gatgaaaggg ttcgatccct accggttagt aatgagttta aacgggggag
     4261 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     4321 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4381 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4441 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4501 tcggggcggc agaccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4621 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4681 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4861 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4921 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4981 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5101 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5161 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5221 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     5281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     5341 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     5401 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5461 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5521 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5581 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5641 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5701 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5761 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5821 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5881 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5941 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6001 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6061 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6121 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6181 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     6241 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6301 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6361 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6481 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6541 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6601 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6661 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6721 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6841 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     7141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7381 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7561 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7621 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7681 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7741 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7801 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7861 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7921 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7981 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8041 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8101 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8161 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8221 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8281 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8341 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8401 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8461 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8521 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8581 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8641 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8701 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
     8761 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     8821 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     8881 g
//
