LOCUS       sequence-71852-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Piatkevich KD, Malashkevich VN, Morozova KS, Nemkovich NA, Almo SC, 
            Verkhusha VV
  TITLE     Extended Stokes shift in fluorescent proteins: chromophore-protein 
            interactions in a near-infrared TagRFP675 variant.
  JOURNAL   Sci Rep. 2013;3:1847. doi: 10.1038/srep01847.
  PUBMED    23677204
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1847. doi"; pages: " 10.1038/srep01847"
COMMENT     Sequence Label: pPaxillin-TagRFP675-N1
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        90..194
                     /gene="bla"
                     /label=AmpR promoter
     promoter        196..553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        196..387
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(219..239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      404..539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(642..661)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
       61 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      181 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
      241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      301 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      361 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
      421 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
      481 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      541 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
      601 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
      661 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
      721 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
      781 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
      841 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
      901 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
      961 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     1021 gtactcggat ggaagccggt cttgtc
//
