LOCUS       sequence-71857-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. 
            Note: This is being tested..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC95-pmax-dCas9VP160-2A-neo
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..43)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(247..266)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
       61 ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt
      121 cgaattctta attaatcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
      181 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
      241 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
      301 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
      361 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
      421 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
      481 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
      541 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
      601 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
      661 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
      721 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
      781 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
      841 gccgattgtc tgttgtgccc
//
