LOCUS       sequence-71864-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP64 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC152-dual-dCas9VP64-sgExpression
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             74..94
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgcctgaaat cacttttttt caggttggac cggtgccacc atgtacccat acgatgttcc
       61 agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg tccgacaaga agtacagcat
      121 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      181 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      241 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      301 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      361 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      421 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      481 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      541 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
      601 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
      661 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
//
