LOCUS       sequence-71865-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP64 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC152-dual-dCas9VP64-sgExpression
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctcaggccg cctctctcgg ccttggtcag attgtcgaac tttctctggg taatcagctt
       61 ggcgttcagc agctgccgcc agtagttctt catcttcttc acgacctctt cggagggcac
      121 gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc accttgttgt cgatggagtc
      181 gtccttcaga aagctctgag gcacgatggc gtccacatcg tagtcggaca gccggttgat
      241 gtccagttcc tggtccacgt acatatcccg cccattctgc aggtagtaca ggtacagctt
      301 ctcgttctgc agctgggtgt tttccacggg gtgttctttc aggatctggc tgcccagctc
      361 tttgatgccc tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc ccttctgggt
      421 ggtctggttc tctctggcca tttcgatcac gatgttctcg ggcttgtgcc ggcccatcac
      481 tttcacgagc tcgtccacca ccttcactgt ctgcaggatg cccttcttaa tggcggggct
      541 gccggccaga ttggcaatgt gctcgtgcag gctatcgccc tggccggaca cctgggcttt
      601 ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc agctgcatga agtttctgtt
      661 ggcgaagccg tcggacttca ggaaatccag gattgtcttg ccggactgct tgtcccggat
      721 gccgttgatc agcttccggc tcagcctgcc ccagccggtg tatctccgcc gcttcagctg
      781 cttcatcact ttgtcgtcga acaggtgggc ataggttttc agccgttcct cgatcatctc
      841 tctgtcctca aacagtgtca gggtcagcac gatatcttcc agaatgtcct cgttttcctc
//
