LOCUS       sequence-71871-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    205..326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(242..261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     296..315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(343..376)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcggcatgga cgagctgtac aagaagctga accctcctga tgagagtggc cccggctgca
       61 tgagctgctg tgtgctctcc tgagcggccg cgactctaga tcataatcag ccataccaca
      121 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      181 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      301 ttgtccaaac tcatcaatgt atcttaaggc gtgtttaaac atataacttc gtataggata
      361 ctttatacga agttatcgcg tacggccacc atggtgagcg agctgattaa ggagaacatg
      421 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
      481 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
      541 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
      601 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
      661 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      721 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
      781 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac cctgtacccc
      841 gctgacggcg gcctggaagg cagagccgac atggccctga a
//
