LOCUS       sequence-71873-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    194..315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(231..250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     285..304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(332..365)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      374..383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctgtaca agaagctgaa ccctcctgat gagagtggcc ccggctgcat gagctgctgt
       61 gtgctctcct gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      121 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      181 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      301 catcaatgta tcttaaggcg tgtttaaaca tataacttcg tataggatac tttatacgaa
      361 gttatcgcgt acggccacca tggtgagcga gctgattaag gagaacatgc acatgaagct
      421 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
      481 gccctacgag ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt
      541 cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac
      601 ccagggcatc cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt
      661 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
      721 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
      781 gcagaagaaa acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg
      841 cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
      901 cttgaagacc acatacagat ccaagaaacc
//
