LOCUS       sequence-71874-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.1
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    35..68
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    85..206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(96..115)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     150..169
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggccgta cgcgataact tcgtataaag tatcctatac
       61 gaagttatat gtttaaacac gccttaagat acattgatga gtttggacaa accacaacta
      121 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      181 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      241 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      301 ctgattatga tctagagtcg cggccgctca ggagagcaca cacttgcagc tcatgcagcc
      361 ggggccactc tcatcaggag ggttcagctt actcacgcgt gcactcttgt acagctcgtc
      421 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
      481 ctcgttgtgg gaggtgatgt ccaacttgat gtcgacgatg taggcgccgg gcagctgcac
      541 gggcttcttg gccttgtagg tggtcttgac ctcggaggtg tagtggccgc cgtccttcag
      601 cttcagcctc atcttgatct tgcccttcag ggcaccgtcc tcggggtaca tccgctcgga
      661 ggaggcctcc cagcccatgg tcttcttctg catcacgggg ccgtcggagg ggaagttggt
      721 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
      781 ggtcaccacg ccgccgtcct cgtagttcat cacgcgctcc cacttgaagc cctcggggaa
      841 ggacagcttg aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
      901 ggtgaaatga ggggacagga tgtcccaggc ga
//
