LOCUS       sequence-71875-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    214..335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(251..270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     305..324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(363..396)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      405..414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acgagctgta caagagtgca cgcgtgagta agctgaaccc tcctgatgag agtggccccg
       61 gctgcatgag ctgcaagtgt gtgctctcct gagcggccgc gactctagat cataatcagc
      121 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      181 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      241 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      301 agttgtggtt tgtccaaact catcaatgta tcttaaggcg tgaagagctt cataatcttc
      361 atataacttc gtatagtata ccttatacga agttatcaag tactgccacc atggtgagcg
      421 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
      481 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      541 tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
      601 tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
      661 ccttccccga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      721 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      781 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
      841 ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga
//
