LOCUS       sequence-71876-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Autobow
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    138..259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(175..194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     229..248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(276..309)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
       61 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      241 aactcatcaa tgtatcttaa ggcgtgttta aacatataac ttcgtatagg atactttata
      301 cgaagttatc gcgtacggcc accatgagca gcggcgccct gctgttccac ggcaagatcc
      361 cctacgtggt ggagatggag ggcaatgtgg atggccacac cttcagcatc cgcggcaagg
      421 gctacggcga tgccagcgtg ggcaaggtgg atgcccagtt catctgcacc accggcgatg
      481 tgcccgtgcc ctggagcacc ctggtgacca ccctgaccta cggcgcccag tgcttcgcca
      541 agtacggccc cgagctgaag gatttctaca agagctgcat gcccgatggc tacgtgcagg
      601 agcgcaccat caccttcgag ggtgatggca atttcaagac ccgcgccgag gtgaccttcg
      661 agaatggcag cgtgtacaat cgcgtgaagc tgaatggcca gggcttcaag aaggatggcc
      721 acgtgctggg caagaatctg gagttcaatt tcacccccca ctgcctgtac atctggggcg
      781 atcaggccaa tcacggcctg aagagcgcct tcaagatctg ccacgagatc accggcagca
      841 agggcgattt catcgtggcc gatcacaccc agatgaatac ccccatcggc ggcggccccg
      901 tgcacgtgcc cgagtaccac cacatgagct accacgtgaa
//
