LOCUS       sequence-71877-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Autobow
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      650..659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    675..708
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     polyA_signal    745..866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     810..829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gccacctcgt gctgctcgac gtaggtctct ttgtcggcct ccttgattct ttccagtctt
       61 ctgtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt ggatctgtat
      121 gtggtcttca agttgcagat caggtggccc ccgcccacga gcttcagggc catgtcggct
      181 ctgccttcca ggccgccgtc agcggggtac agggtctcgg tggaggcctc ccagccgagt
      241 gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat cttgacgttg
      301 tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac gcccccgtct
      361 tcgtatgtgg tgactctctc ccatgtgaag ccctcgggga aggactgctt aaagaagtcg
      421 gggatgccct gggtgtggtt gatgaaggtt ttgctgccgt acatgaagct ggtagccagg
      481 atgtcgaagg cgaaggggag agggccgccc tcgaccgcct tgattctcat ggtctgggtg
      541 ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt gttcacggtg
      601 ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcac catggtggca
      661 gtactggata cttgataact tcgtataagg tatactatac gaagttatat gaagaacgcg
      721 aagattatga agctcttcac gccttaagat acattgatga gtttggacaa accacaacta
      781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      841 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
//
