LOCUS       sequence-71878-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of scrambled shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
  TITLE     SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 
            signaling.
  JOURNAL   Mol Cell. 2012 Jun 29;46(6):833-46. doi: 
            10.1016/j.molcel.2012.04.007. Epub 2012 May 9.
  PUBMED    22575674
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.04.007"; journalName: "Mol Cell"; date: 
            "2012-06-29- 29"; volume: "46"; issue: "6"; pages: "833-46"
COMMENT     Sequence Label: pLKO-sh-hSC
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    301..418
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      181 tcttgtggaa aggacgaaac accggtaacg tacgcggaat acttcgactc gagtcgaagt
      241 attccgcgta cgtttttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa
      301 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      361 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      421 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
      481 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      541 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      601 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      661 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      721 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc ccgaccgcga
      781 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
//
