LOCUS       sequence-71879-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of shRNA against human SH3BP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
  TITLE     SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 
            signaling.
  JOURNAL   Mol Cell. 2012 Jun 29;46(6):833-46. doi: 
            10.1016/j.molcel.2012.04.007. Epub 2012 May 9.
  PUBMED    22575674
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.04.007"; journalName: "Mol Cell"; date: 
            "2012-06-29- 29"; volume: "46"; issue: "6"; pages: "833-46"
COMMENT     Sequence Label: pLKO-sh-hSH3BP4
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    300..417
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgggatgaa agtgtcagcc gagatctcga gatctcggct
      241 gacactttca tcttttttga attctcgacc tcgagacaaa tggcagtatt catccacaat
      301 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      361 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      421 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
      481 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
      541 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
      601 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
      661 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
      721 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
      781 ggctgtggcc aatagcggct gctcagccag gcgcgcccg
//
