LOCUS       sequence-71885-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Cyprinodon variegatus cryaa
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             554..571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(624..642)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      638..685
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(809..828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttagaagga gatatacata tggatattgc catccagcac ccctggttta gacgtgccct
       61 gggctccacc tatcctgccc gactctttga ccaatttttc ggagagggaa ttttcgacta
      121 tgaccttttc cccatcacca ccaccatcag tccctattat agacactcgc tgttccgcaa
      181 tcttctggat tcctccaact ccgggatctc tgaggtgagg tctgacaggg acaagtttac
      241 agtctacctg gatgtcaaac acttctcccc tgatgagctc agtgtgaagg tgactgatga
      301 ctacgtggaa atccagggca agcatggaga aagacaggat gaccatggtt acatctctcg
      361 cgagttccat cgccggtacc gtctgccctc cgctgtcgac caatcagcca tcacctgtac
      421 cctgtcagct gacggtctgc tgactctgac tggtccaaag gtgtccggag ggagtgaatc
      481 tggtcggggt gatcgcagca tccccgtcac ccgcgatgac aagaccagcg ctgctccatc
      541 ctcctagctc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      601 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      661 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      721 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      901 agtgctttac ggcacctcga cc
//
