LOCUS       sequence-71886-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Dissostichus mawsoni cryaa
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             594..611
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(664..682)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      678..725
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(849..868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaaggagat atacatatgg aaatttctat ccagcatccc tggttcagac gggccatggg
       61 ctccgtctac cccgccagac tctttgacca gttcttcgga gagggcatgt ttgactacga
      121 cttcttcccc tacaccacct ccaccatcag cccctactac agacagtccc tgttccgaag
      181 cttcttggat tcctcaaact ccggcacctc tgaggtgagg tctgacaggg ataagtacac
      241 tttgtacctg gatgtcaaac acttctctcc tgaagagatc agtgtgaagg tgaccgggga
      301 ctacgtggag atccagggca agcatggaga gagacaggat gaccacggtt acatctctcg
      361 tgagttccac cgccgctacc gcctcccctc cagtgtggac caatcagcaa tcagctgtag
      421 cctgtcagct gacgggctgc tgaccctgag cgggccaaag gtcaacgggg ggagcgaatc
      481 cggccgcagc gagcgcagca tccctgtcac ccgcgacgag aagaccaact ctgctgtgtc
      541 ctcctaggga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc
      601 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      661 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      721 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      841 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctt
//
