LOCUS       sequence-71902-e        8369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of the
            blue fluorescent protein AmCyan and the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1835..1952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1999..2032
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        2064..3275
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3112..3131
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3307..3993
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             4012..4068
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4090..4143
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    4165..4198
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    4210..4798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4263..4283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4681..4692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4889..5122
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5194..5315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5231..5250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5285..5304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5355..5490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5510..5529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5511..5529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5539..5556)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5539..5555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5548..5570)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5700..6155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5787..5806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5997..6018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6184..6288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6289..6357,6358..7149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6507..6526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7320..7908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7809..7828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8062..8079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8196..8217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8232..8237,8238..8255,8256..8262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8270..8286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8275..8297
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8294..8310
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8294..8310
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8329..8349
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8331..8349
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     1801 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     1861 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     1921 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     1981 cagtttggtt aattaacgat aacttcgtat aatgtgtact atacgaagtt atctcgagat
     2041 taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     2101 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     2161 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     2221 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2281 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2341 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2401 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2461 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2521 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2581 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2641 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2701 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2761 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2821 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2881 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2941 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     3001 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3061 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3121 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3181 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3241 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtccgatcc
     3301 accggtatgg ccctgtccaa caagttcatc ggcgacgaca tgaagatgac ctaccacatg
     3361 gacggctgcg tgaacggcca ctacttcacc gtgaagggcg agggcagcgg caagccctac
     3421 gagggcaccc agacctccac cttcaaggtg accatggcca acggcggccc cctggccttc
     3481 tccttcgaca tcctgtccac cgtgttcatg tacggcaacc gctgcttcac cgcctacccc
     3541 accagcatgc ccgactactt caagcaggcc ttccccgacg gcatgtccta cgagagaacc
     3601 ttcacctacg aggacggcgg cgtggccacc gccagctggg agatcagcct gaagggcaac
     3661 tgcttcgagc acaagtccac cttccacggc gtgaacttcc ccgccgacgg ccccgtgatg
     3721 gccaagaaga ccaccggctg ggacccctcc ttcgagaaga tgaccgtgtg cgacggcatc
     3781 ttgaagggcg acgtgaccgc cttcctgatg ctgcagggcg gcggcaacta cagatgccag
     3841 ttccacacct cctacaagac caagaagccc gtgaccatgc cccccaacca cgtggtggag
     3901 caccgcatcg ccagaaccga cctggacaag ggcggcaaca gcgtgcagct gaccgagcac
     3961 gccgtggccc acatcacctc cgtggtgccc ttcgtgtaca agggatctgg agcaacaaac
     4021 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccgggccttc tagaggatcc
     4081 ggctccggcg agggcagggg aagtctacta acatgcgggg acgtggagga aaatcccggc
     4141 ccagctagcg tgtgatcaga attcataact tcgtataatg tgtactatac gaagttatgt
     4201 cgactcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4741 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4801 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4861 agccactttt taaaagaaaa ggggggactg gaagggctac gtaactccca acgaagacaa
     4921 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4981 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5041 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5101 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5161 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     5221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5281 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     5341 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5401 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5461 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     5521 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5581 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5641 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
     5701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6001 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6121 cgcgaatttt aacaaaatat taacgtttac aatttcccag gtggcacttt tcggggaaat
     6181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8101 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8161 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8221 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8281 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     8341 cactaaaggg aacaaaagct ggagctgca
//
