LOCUS       sequence-71918-e        3857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish ets1a gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 374 pME-ets1a
FEATURES             Location/Qualifiers
     source          1..3857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1982..2081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2098..2117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2099..2117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2122..2138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2240..2259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2251..3060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2318..2337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3207..3795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3696..3715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc gtgaccatga cggcagctgt cgatattaag ccgttaacta taataaaatc
      721 cgaaaaagta gacgatctgg agtgtgctga tgttcctctt ctgactccgg gaagtaagga
      781 aatgatgtca caagctcttc tggccacatt cagcggcttc accagagaac agcagagact
      841 ctccatcccc aaagacccgc gtgagtggac agaaggccat gtgagggaat ggttgacctg
      901 gacggtgaat gagttcagtc tgaagaacgt ggacttccac aagttcagta tggacggagc
      961 gagtctctgt gcgctcggca aggagagatt tctggacctg gcaccagact ttgtgggcga
     1021 catcctctgg gggcatttag agatgctgca gaaagaagac ccgaagcact tccccgtcag
     1081 cagcctgagc tccagcttcc aggagtcccg ctatccctcc gaatatttct tcaactatgg
     1141 cattgagcat cctcagtgtg tccctccgtc tgaatactct gagccgagct tcatcacaga
     1201 gtcctatcag acgcttcacc ccatcagttc agaagacctg ctgtcgctca aatacgagag
     1261 cgagtatccc aacgtcatcc tgcgggacgc gccgctcaac ccmctgcagg gagactattt
     1321 ctcagtcaaa caggaagtcg tgtcgcctga caacatgtgt gtgggtcgca ttagcagagg
     1381 taagctgggy ggtcaggact cgtttgagag catcgacagt ttcgagagct gcgacagact
     1441 cacgcagtcg tggagcagtc agtcgtcctt caacagcctt cagagagtcc cgtcctatga
     1501 cagcttcgac tcggaggatt accccagtgc tctgcatgcr cacaaaccca agggcacgtt
     1561 taaagactac gtgcgggaac gctcggacct gagcaaagac aaaccggtca tccccgcggc
     1621 ggctctcgct ggatacacag gcagtggacc catccagctc tggcagttcc tgttggagct
     1681 cctgacggat aaatcctgcc agtccttcat cagctggact ggagacggct gggagttcaa
     1741 gctttctgac ccggatgagg tggcccgcag atggggaaag aggaaaaaca agcccaagat
     1801 gaactatgaa aagctgagcc gcggcctgcg ctactactac gacaagaaca tcatccacaa
     1861 aacgtccggc aagcgctacg tctaccgctt cgtgtgcgac ttgaaaagcc tgctgggata
     1921 cactcccgag gagctgcaca ccatgttaga cgtcaagccc gacacggacg agtaaagtct
     1981 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2041 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2101 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2161 aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct
     2221 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2281 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2341 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2401 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2461 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2521 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2581 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2641 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2701 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2761 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2821 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2881 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2941 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3001 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3061 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3121 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3841 ccttttgctc acatgtt
//
