LOCUS       sequence-71925-e        3908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish fli1a gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 375 pME-fli1a
FEATURES             Location/Qualifiers
     source          1..3908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2033..2132)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2149..2168)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2150..2168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2173..2189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2173..2189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2291..2310)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2302..3111
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2369..2388)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3258..3846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3747..3766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttg cgcaaaatgg acggaactat taaggaggcg ctgtcagtgg tgagtgaaga
      721 ccagtctctg ttcgagcctc catacgccgc tgctgctcct ttacccaaga cagacatgac
      781 tgcgtccggg actcaggatt acggccagac gcacaaaatc aaccccattc cccctcagca
      841 agagtggatt aatcagccgg tgcgggtgaa cgtcaagcga gagtatgacc acatcaacgg
      901 atccagagag tcgccggtgg actgcagcgt ggggaaatgc aataaaatgg tgggcgggac
      961 cgaagcgtct cagatgaact atactggcta catggatgag aaatgtgctc cgcctccaaa
     1021 catgacgacc aatgagagga gagtcatcgt ccccgcagac ccgtctctgt ggtctccgga
     1081 tcacgtgcgg cagtggttag actgggccat taaggagtac ggtcttcagg agatcgacac
     1141 ggccatgttt cacagcacag atggaaaaga gctctgcaag atgagcaaag acgacttcct
     1201 gagactcacc agcgtttata acaccgaggt ccttctctca catctcaatt acctcaggga
     1261 aagtagctca tcgatatcat acaacacgcc atctcacgct gaccagtccc ctcgcctggc
     1321 tgcaaaagac gatgcgtctt atgatgctgt acggaggacg ggctggtcga acaacatgca
     1381 cagcggaaaa ggctctccaa cagtggtctc tcagagcgtg tccaaaaacc cggatcagcc
     1441 tcgtcctcag ccagatcctt atcagatatt aggacccacc agcagtcgac ttgctaaccc
     1501 aggctcaggt cagatccagc tgtggcagtt cctgctggag ctcctgtccg acagcgccaa
     1561 cgccggctgc atcacctggg aaggaaccaa cggagagttc aagatgacgg acccagacga
     1621 ggtggcacgg cgctggggcg aacgcaagag caaacccaac atgaactacg acaagctgag
     1681 ccgagccctg cgctactact acgacaagaa catcatgacc aaagtgcacg gcaagcgcta
     1741 cgcctacaag ttcgacttcc acggcatcgc ccaggccctg caaccccatc ccaccgagtc
     1801 caccatgtac aagtacccaa gcgagctgcc gtacgtgcct agctatcacg cccaccagca
     1861 gaaggtcaac ttcgtctctc ctcaccctcc gtcaatgccc gtcacctcct ccaacttctt
     1921 cggtcccaca accccatact ggagctcccc accaggaggt atttacccca atcccagcgt
     1981 cccacgccat gccaattcac acgtgccttc acaccttggt agttactact aaacccagct
     2041 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2101 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2161 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2221 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata
     2281 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2341 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2401 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2461 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2521 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2581 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2641 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2701 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2761 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2821 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2881 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2941 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3001 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3061 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3121 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3181 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3901 cacatgtt
//
