LOCUS       sequence-71927-e        3650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish etv2 gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 459 pME-etv2
FEATURES             Location/Qualifiers
     source          1..3650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1775..1874)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1891..1910)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1892..1910)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1915..1931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1915..1931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2033..2052)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2044..2853
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2111..2130)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3000..3588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3489..3508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta accatggaaa tgtaccaatc tggattttac acagaagact tcagaactca
      721 ggaggttcct gctggtttcg acttcagttc atatgactgc agtggtgaag acctgtcctt
      781 tttattagac agcaaaggac ccgtccaaca gcagtatgcc gaaaactact ccgagcctca
      841 gaaggagctg ttgcacaaag gtcatgttct cacagtcgac tctgggctgt tcaatctgga
      901 ctctttccct gagttcagca actgggcagc atacactaat attccagaag gaatggttgc
      961 agacagacag caggttggct ttcaggaatc aactcaaacc taccagaacc tcgtccctct
     1021 ctgcactcca gcacaaagca gtacattcag tcccacaatg gacaccagca gccattacca
     1081 acctgggaaa ggcccaagtc acagaggagc atccgggacc gccagccttg accatttggg
     1141 tgaatcagac agaacatatg gtttgtatga agcagagcag caaagcaggt cttcatattg
     1201 gtccgactac ccctcacccg gttactgcag ctccatgccg ctgagccagc ctgcatcctc
     1261 ctcatcccct ccggtcagtc agtccgctga gcacttctgc ccccgcgtgg tcaaaagacg
     1321 cagcgctcct cctcaaagat cagacagaga gggcgagatc acgccaatgt ccgcctaccc
     1381 aggatctgga cccatccagc tttggcagtt tctgctagaa ctcctgctgg attctgcttg
     1441 ccacactttt ataagttgga ctggtgatgg ctgggagttt aaaatgtcag atcccgctga
     1501 ggtggcgaag cggtggggcc agtgtaaaaa caagcctaaa atgaactatg agaagctcag
     1561 tcgtggcctg cgttactact accacaaaaa catcattcac aaaacggcgg gaaagcgcta
     1621 cgtctaccgc tttgtctgtg acgtgcaggg catgcttgga aaaactgcac acgaagtctt
     1681 agcaagtcta aacatctccc cgaatgcagc atctccacag tctgtagcaa acacaagccg
     1741 ctcagaggaa accacagagt cctggacaca ttagacccag ctttcttgta caaagttggc
     1801 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1861 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1921 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1981 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2041 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2101 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2161 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2221 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2281 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2341 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2401 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2461 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2521 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2581 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2641 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2701 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2761 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2821 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2881 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
