LOCUS       sequence-71931-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression empty vector (yeast).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_gRNA_handle_RPR1t
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..411
                     /gene="RPR1"
                     /label=RPR1 promoter
                     /note="promoter for the S. cerevisiae RNase P RNA gene"
     misc_RNA        419..494
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      507..569
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
ORIGIN
        1 gggggatctg ccaattgaac ataacatggt agttacatat actagtaata tggttcggca
       61 cacattaaaa gtataaaaac tatctgaatt acgaattaca tatattggtc ataaaaatca
      121 atcaatcatc gtgtgtttta tatgtctctt atctaagtat aagaatatcc atagttaata
      181 ttcacttacg ctacctttta acctgtaatc attgtcaaca ggatatgtta acgacccaca
      241 ttgataaacg ctagtatttc tttttcctct tcttattggc cggctgtctc tatactcccc
      301 tatagtctgt ttcttttcgt ttcgattgtt ttacgtttga ggcctcgtgg cgcacatggt
      361 acgctgtggt gctcgcggct gggaacgaaa ctctgggagc tgcgattggc agaagcttgt
      421 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      481 caccgagtcg gtgctttttt ctcgagccat atccaacttc caatttaatc tttctttttt
      541 aattttcact tatttgcgat acagaaagaa aaaagcgata gtaactattg aattttgttt
      601 ggatttggtt agattagata tggtttctct ttatatttac atgctaaaaa tgggctacac
      661 cagagataca taattagata tatatacgcc agtacacctt atcggcccaa gccttgtccc
      721 aaggcagcgt tttgttcttg gaaacgctgc cctacacgtt cgctatgctt caagaacttt
      781 tctgagcact tcatgatgca tgtttgttcc ttattggtta gctttgatgt tgtgaagtca
      841 ttgacacagt ctgtgaaaca tctttctacc agattagagt acaaacgcat gaaatccttc
      901 atttgctttt gttccactac tttttggaac tcttgttgtt cttt
//
