LOCUS       sequence-71935-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-5-G4BDMD-QFADM1-hsp70
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    245..314
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagctggc tagaactagt gtcgacatgc
       61 ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc gccgacgccg
      121 tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc gagggcttcc
      181 cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc atcatgatgg
      241 accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc tggcacccgc
      301 accgcggctt cgagaccgtg acctacatcg tcgacactag tggatctcta gaggtaccct
      361 cgagccgcgg ccgcggatct aaacgagttt ttaagcaaac tcactccctg acaataaaaa
      421 cgcaattatc tttaaataaa tatattctat ttattaacca agtagctaat atagctaaaa
      481 tcaatttgtt gctaacttaa aactaacata attatgtatt agttatttac attcttttgg
      541 ttggttacaa ccaaataaat aatatcactt ttaaaaactt aagccaggaa ctgaataatt
      601 ttctatttgg ctttagtcga cggtatcgat agacgtcatc atttcttctt tttgtatgta
      661 ttaatgtcgg agaagttaca tccaggaacg aatcccagat tggtcataaa ctgtgggtcg
      721 acatccacag cgtccgtgta ttcaatgcta gccaaatcgt caaccatggc atcgtaatca
      781 acggtcgagt aaccaaagtt cgccattgga accagattcg agtggtacgg cgggtgattg
      841 gtcgtgctgt caacatacgg agga
//
