LOCUS       sequence-71940-e        4585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish lnx1 gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 666 pME-Lnx-1
FEATURES             Location/Qualifiers
     source          1..4585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2712..2811)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2828..2847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2829..2847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2852..2868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2852..2868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2970..2989)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2981..3790
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3048..3067)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3937..4525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4426..4445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca accatgacgg agtctaagac gtcttcactg ccgaccggtg taacgtgggc
      721 gcgggtgtgt aaggaatgtg gacagtgcca tgatggccaa gacagtcatc tgtttgaata
      781 tcaggatgag gtggatgatg agctggtctg ccacatctgc ctacaacctc tgttgcagcc
      841 tatggacaca ccgtgcggcc acacttattg cttccagtgc ctcagcaatt tcctgcacga
      901 ccaggacttc tgtcctgtcg accgccaaag gattcagcta caacaatgca gagcctccag
      961 cctgctggta cgcaatctgc tggacaaact ggccgtgctg tgcccgtttc gcagtgagtg
     1021 cgagctgagc atgcaacgtt gtgagttgca acctcatctg cacaacagat gccctgcgtt
     1081 caagcgactc agggaggagg cggagagaaa aaagagaccc tcctggaatg agttaaaggg
     1141 aagtaaatca gatggagatt tagctgatgc caaatctgcc caaacaatcg gacgcacagc
     1201 tcagctcagc gcaccctcag aacctggact ggtcaacccc gccttcgagg agggcgaaga
     1261 tgacacccct ctgaggtgca gtcttgtggc tgaggcgact gtggtggagc tgtttcgtga
     1321 agatccaggc gaggatttgg gattgcgtat agtgggagga aaagacacac ccttgggcaa
     1381 catcgtcatt caggagatcg tgcgtgactc actggtggcc cgagatggca aactggcacc
     1441 tggagatcat atcctggagg tgaatgatgt cagtctggca tctatctctc acagtcgtgc
     1501 cattgctgtg attcggcagc cgtgttcccg cctcaggctc actgtcatgc aggagaaggg
     1561 cttcaaaccc cggcccgaac atcacactca gccctcagct tcacctccaa cacagtcacc
     1621 cagtaccaac cagaaccacg gtactgtcat ccaggtcacc ctggtgaagc atgagcgttc
     1681 agaggcgctt ggcatcaaac tcatccgcaa gtctgaggag ccaggagttt tcatcctgga
     1741 ccttctccct ggaggactgg cggccaaaga tggcaaattg aggaacaacg ataaagtgct
     1801 gggcattaat ggacaggacc tgcgacatgg caccccagag agtgcagctc agatcataca
     1861 ggcaagtgaa atgcgtgtga actttgtagt gatgaggctt caagatgtct ccgaggaggg
     1921 tggtgaggga cagagcagag gggcccggag agtccccgaa ccacagtatt tcagacgcca
     1981 ctctgaatac atgaaggagc ctccaggcgg attctccagt caagagaaga ctgtgaccct
     2041 aaagaaggag cctcggcagt ctctaggcat caccattgca ggtggcagag actgtcgcag
     2101 ccgtttgcct gtctacatca ctagtgttca gcctgtgggc tgcctgcaca gagacggcac
     2161 tgtcaaaaca ggtgatgttc ttttgagtat aaatggtatt gacctgaccc atctgaccta
     2221 caacgaggca gtgactgtcc tgaagaccca gactgctcaa agcacagtaa cgctgcgagt
     2281 catccagacg tttgctgatg atgaagaaca ggatggagaa tctggcagta gagaagacat
     2341 ggacacaatg gagggcccca aagatgatga catcaactgg gttcctctct ggacccgctg
     2401 gctcgggctg cccagttgca tccactggtg tcgagatata atgctgatga agaccaacag
     2461 tgagagctgg ggcttcagta ttgtgggagg atatgaggag agtcgtggcc aacagccctt
     2521 cttcatcaag accattgtgc ctggaacacc agcttacttt gacggacgcc tcaagtgtgg
     2581 tgatgagatt gtggcagtga atggagtcac tacagtgggc atgaataact cctccttgat
     2641 acccatgctg aaactgcaga agaacaaagt cactctgacc gtggtctcct ggcctggcag
     2701 tctggtttaa tacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2761 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2821 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2881 cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat
     2941 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3001 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     3061 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     3121 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3181 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3241 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     3301 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3361 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3421 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3481 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3541 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3601 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3661 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3721 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3781 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3841 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4561 cttttgctgg ccttttgctc acatg
//
