LOCUS       sequence-71943-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6-MBP TEV Ura S. cerevisiae express...
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..126
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             173..190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta
       61 cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt
      121 aacgtcaagg agaaaaaacc ccggatccgt aaccatgtca actggttctt ctcaccatca
      181 ccatcaccat ggttcttcta tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      241 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      301 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      361 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      421 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      481 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      541 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      601 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      661 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      721 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      781 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      841 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      901 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
      961 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1021 agagctggca aaag
//
