LOCUS       sequence-71945-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Danio rerio cryaa T147V
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             583..600
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(653..671)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      667..714
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaggagatat acatatggat attgcgatcc aacacccttg gttcagacgc acactgggct
       61 accccacccg actctttgat cagttctttg gagaaggcct gttcgattat gacctattcc
      121 ccttcaccac ttcaactgtc agtccttact atcgacactc actcttccgc aacatcctgg
      181 actcctccaa ctcaggtgtc tctgaggtga ggtctgacag agaaaaattt acagtttatc
      241 tggatgtgaa acatttctct cctgatgagc tcagtgtcaa ggtgacagat gactatgtgg
      301 agatccaggg caagcatgga gaaagacagg atgatcatgg ctacatctcc cgtgagttcc
      361 atcgtcgcta ccgcctgcct tccaatgtgg accagtctgc catcacctgc acactgtctg
      421 ctgatggcct gctcactctt tgtggaccca aggttagtgg catagatgct ggccgtggag
      481 atcgcaccat ccctgttacc cgcgaggaca agagcaactc aggctcttcc tcctagggat
      541 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      601 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      661 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      721 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
//
