LOCUS       sequence-71946-e        3679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 1208 p3e-long etv2 3'UTR
FEATURES             Location/Qualifiers
     source          1..3679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1788..1883)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1920..1939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1921..1939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1944..1960)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1944..1960)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2062..2081)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2073..2882
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2140..2159)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3029..3617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3518..3537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggagg aggaattctc gaaggattgg aaagaacttt
      781 aaaacatcag acgtcgtgcc tttggaggaa gaaagaaatg actgacttga gatttatgta
      841 ctttaatttg gactttgcag cagattgcca acaacagatc tgaagtcagc tttgaaatca
      901 agctaggtgc ctttagaaaa ccatgttagc tacctctttt cacattggaa cattaatctt
      961 gtaaatgtat tttgttttat cttatgtaaa ctgatggttt gacatcagat agtgaactct
     1021 tgtaaataaa tgtaatatga cagcttcact tttcaatatg tccttattta tatatgcgtg
     1081 ttgaagacgg acgattacat ttttaaaagt ttgctctata tcaggggtgt ccaaacttgg
     1141 tcctggaggg ccgatgtctt gcttattttg gttccaaacc catttcaaca cacctgaacc
     1201 agttaatcaa ctctttctag gtaggggaga ccgggtatag ttgaaacaca aggagagttg
     1261 taacgctacc aattccagga atagggaata agataggggt catgtgacac tttcagtttc
     1321 ttcaggtttt cttaacaatc acatgtggag actttccagt caggcttgca atctctccaa
     1381 aagcttcaac agaaaatcga attctgaggt ttcaaaaaaa aaaaaaaaaa tagcaagata
     1441 ttattttgtt tagtacctct accgttgtag atgatgttgt atgagttata tcaggtcaaa
     1501 ctgtattttc tctagtaaag aatggtgtat caacctcctg ctaatttcaa agcaccatgc
     1561 ttaaacagct agctaaacaa tgggtgacag tggcggggta ggttgaaaaa cgtgttttga
     1621 aagtatacat gcacttaaac acacacatga atgtgcacgt atagacaagc acacacaaac
     1681 acacgtgcat gcacacacac acacacaata agcacataca cttacaatat tcacatatat
     1741 tgtattgttg cagtcattta tttacaataa aactgttgtt gtggcatcaa ctttattata
     1801 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1861 cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc
     1921 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1981 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     2041 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     2101 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2161 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     2221 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2281 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2341 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     2401 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2461 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2521 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2581 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2641 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2701 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2761 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2821 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2881 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2941 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3001 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3061 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3421 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3661 ggccttttgc tcacatgtt
//
