LOCUS       sequence-71948-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Danio rerio cryabb
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             596..613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(666..684)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      680..727
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggacatc gccatcaatc ccccgttccg
       61 gcgcatctta tttcctatct tctttccccg ccggcaattt ggggagcata ttactgaggc
      121 cgacgtgatc tcctcattgt actcccaacg atcttccttt cttcgcagtc caagttggat
      181 ggaaagcgga gtttctgagg tgaaaatgga aaaggaccaa ttctcactaa gtctggatgt
      241 taaacacttt gcaccagagg agctatcagt gaaaatcatt ggagatttca ttgaaattca
      301 tgctaaacat gaagatcgtc aggacggtca cggttttgtc tcacgagagt ttttgagaaa
      361 ataccgtgtc cctgttggtg tggatccagc ctccatcacg tcctcgctgt cttctgatgg
      421 tgtgctgaca gtgacaggcc cactaaagct ctcagacggc ccagaacgca ccatcgccat
      481 ccccgtaact cgtgatgaca aaaccaccgt cgctggccct cagaagtaaa gatgctaaac
      541 gtcaactttc aaaaaaaaaa aaaaaaaggg cggccgctct agagtatccc tcgagcacca
      601 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      661 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      721 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tg
//
