LOCUS       sequence-71954-e        4031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish fli1b gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: pME-fli1b
FEATURES             Location/Qualifiers
     source          1..4031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2274..2293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2275..2293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2298..2314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2298..2314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2416..2435)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2427..3236
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2494..2513)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3383..3971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3872..3891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca cagaaatcgg caatggactg tactattaag gaagctctat cggtggtgag
      721 tgaagaccag tccatattcg agtcgccctt cagtggtccc caggccatgc acatgaaggc
      781 ggagatcggc tcgcctgcgg tgttcagaca ggcttcagaa gagagcatcg aacccactga
      841 accagactgg agcggatcgg cagcacaaaa ctctgccaaa agagcagaac acatcaacgg
      901 gaccagccgt gagtccccag tggactgtag tgtgactaaa aggactcgac acatgagtaa
      961 cagtgatgga ggacagttgt cttaccaggc ctcgtaccca gagccccgca gcagcccaca
     1021 gacggccaca ccacccagca gcaccacaga ggagaagagg gtcatcgtac cagccgaccc
     1081 agaggtttgg acacaagacc acgtgcggca gtgggtggac tgggccataa aggaatacgc
     1141 gctgtccgat gtggacgtgt ccctcttcca gaccctggat gggaaagcgc tctgcaaatt
     1201 gagcaaagag gacatgatgc gcatcacctc cgcatacaac acagacatcc tgctctccca
     1261 tctcaactac ctccgggaga gtagtcccac tttttcctac ccaacaaatc caactaatac
     1321 acaaccacaa cctcgagtac aggtgaaagc cgcagaaaac agctttgagg agatcggcag
     1381 gagaaacagt tggtcctcaa gtggattgcc agctgtccct aaaggatctc caattgaaca
     1441 tcagcacaat accagaatta cagagccgcc tccaagactt ccacaagacc cctaccaagc
     1501 attaggttcc atcagcagtc gtcttgcaaa cccagaagcg aaaccaatcc acaccaagaa
     1561 ccgaaccacc aaacaaaatt ctagccatct gcctgtttcc agcactgaca ggactgtggg
     1621 ctctggtcag atccagctgt ggcagttctt actggagttg ctttccgaca gcaacaactc
     1681 gaccatcatc acctgggagg gaactaatgg ggaattcaag atgacagacc ctgacgaggt
     1741 ggccaaacgc tggggggagc gaaaaagcaa gcctaacatg aactacgaca agctcagccg
     1801 tgcccttcgc tactactacg acaaaaacat catgaccaaa gtgcacggca aacgctacgc
     1861 atacaaattc gacttccagg gcatctccca aagtcaccaa aaccactcca cagagggaag
     1921 cgtgtataaa ttccagaccg aagggtcgta catgcagagc taccacagcc accagccaaa
     1981 aatgaacttc ataagcccac attccacccc tatgtctgtt acaactagta atttcttcac
     2041 gcctcaatcc acctactgga actccgcaac cagtgtggtt tatcccagtt caccgatgcc
     2101 acgacatccc agcactcaca ctcacttgaa cacttattat taaacccagc ttcttgtaca
     2161 aagtggtctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2221 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2281 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2341 tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt
     2401 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     2461 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     2521 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     2581 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     2641 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     2701 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     2761 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     2821 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     2881 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     2941 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     3001 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     3061 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     3121 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     3181 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     3241 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     3301 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     3361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4021 ttgctcacat g
//
