LOCUS       sequence-71956-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Zhao N, Liu H, Lilly B
  TITLE     Reciprocal regulation of syndecan-2 and Notch signaling in vascular 
            smooth muscle cells.
  JOURNAL   J Biol Chem. 2012 May 11;287(20):16111-20. Epub 2012 Mar 21.
  PUBMED    22437834
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2012-05-11"; volume: "287(20)"; pages: "16111-20. 
            Epub 2012 Mar 21"
COMMENT     Sequence Label: hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..762)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      783..792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta gcgagatccg gtggagccgg gtccggcggt
       61 gccgtccacg gcagaattgg acgactgagc gcgggatctg gcgaaggcaa tgggggtctt
      121 gaaggcgtgc tggtactcca cgatgcccag ctcggtgttg ctgtgcagct cctccacgcg
      181 gcggaaggcg aacatggggc ccccgttctg caggatgctg gggtggatgg cgctcttgaa
      241 gtgcatgtgg ctgtccacca cgaagctgta gtagccgccg tcgcgcaggc tgaaggtgcg
      301 ggcgaagctg cccaccagca cgttatcgcc catggggtgc aggtgctcca cggtggcgtt
      361 gctgcggatg atcttgtcgg tgaagatcac gctgtcctcg gggaagccgg tgcccaccac
      421 cttgaagtcg ccgatcacgc ggccggcctc gtagcggtag ctgaagctca cgtgcagcac
      481 gccgccgtcc tcgtacttct cgatgcgggt gttggtgtag ccgccgttgt tgatggcgtg
      541 caggaagggg ttctcgtagc cgctggggta ggtgccgaag tggtagaagc cgtagcccat
      601 cacgtggctc agcaggtagg ggctgaaggt cagggcgcct ttggtgctct tcatcttgtt
      661 ggtcatgcgg ccctgcttgg gggtgccctc tccgccgccc accagctcga actccacgcc
      721 gttcagggtg ccggtgatgc ggcactcgat ctccatggcg ggcaggccgc tctcgtcgct
      781 ctccatggtg gcgtctagcg taggcgccgg tcacagcttg gatctgtaac ggcgcagaac
      841 ag
//
