LOCUS       sequence-71958-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Trp S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..41)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    414..461
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
       61 ataataatgg tttcttagta tgatccaata tcaaaggaaa tgatagcatt gaaggatgag
      121 actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc
      181 atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc
      241 tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat gccgttcttc
      301 tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt
      361 atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc
      421 ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaaaacca
      481 aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac
      541 taaaataatg cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat
      601 ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga acttgcatct
      661 aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt
      721 gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat
      781 acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat
      841 catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata
      901 atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg
      961 cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt
     1021 ctaaccttaa cggaccta
//
