LOCUS       sequence-71966-e        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Trp S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     80..96
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     80..96
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        383..1047
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    599..716
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1011..1034
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1081..1098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgct ctagaactag tggatctttt gaaagatgat actctttatt
      121 gcagccagtt atataagatc ttttgaaaga tgatactctt tattgcatgc cagttatata
      181 agatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga ccaattcgac
      241 aggttatcag caacaacaca gtcatatcca ttctcaatta gctctaccac agtgtgtgaa
      301 ccaatgtatc cagcaccacc tgtaaccaaa acaattttag aagtactttc actttgtaac
      361 tgagctgtca tttatattga attttcaaaa attcttactt tttttttgga tggacgcaaa
      421 gaagtttaat aatcatatta catggcatta ccaccatata catatccata tacatatcca
      481 tatctaatct tacttatatg ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa
      541 aaccttctct ttggaacttt cagtaatacg cttaactgct cattgctata ttgaagtacg
      601 gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct
      661 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
      721 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
      781 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
      841 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
      901 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
      961 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
     1021 tatactttaa cgtcaaggag aaaaaacccc ggatccgtaa ccatgtcaac tggttcttct
     1081 caccatcacc atcaccatgg ttcttc
//
