LOCUS       sequence-71970-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Trp S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..128
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             175..192
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             202..222
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     794..815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt cggtttgtat
       61 tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact
      121 ttaacgtcaa ggagaaaaaa ccccggatcc gtaaccatgt caactggttc ttctcaccat
      181 caccatcacc atggttcttc tgaaaacctg tacttccaat ccaatattgg aagtggataa
      241 ctctcgagga tcgtaatgac tagattatat aatcagaaat agcctttggt gatcacttcc
      301 attttagtca gcataataga aagtttaatt acgcgctttt ttacattccg acaaagatct
      361 gcccgagaaa attcttcgag aagacaacaa caacaaaaaa aataaagcct gggcgaaaaa
      421 catcgtgcgt ttacataata tcttgaaaag caaacagata tataaaccgc tattgaaatt
      481 acctttttaa gtattcgcct agaagccttg tttcaagtag catcaacaac aggtgttagg
      541 ttaaactgct ttgacttttt gttctttaac gaatatcatt aaaaaaaaag gacatctata
      601 aagaacgcgt aattcctgca gcccggcttt ccccgtcaag ctctaaatcg ggggctccct
      661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      901 atttaacaaa aatttaacgc gaattttaac aaattattaa cgtttacaat ttcctgatgc
      961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagatcgg caagtgcaca
     1021 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     1081 ttgctatttt gttaga
//
