LOCUS       sequence-71974-e        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6-MBP TEV Trp S. cerevisiae express...
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..129
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             176..193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atataaatgc aaaaactgca taaccacttt aactaatact ttcaacattt tcggtttgta
       61 ttacttctta ttcaaatgta ataaaagtat caacaaaaaa ttgttaatat acctctatac
      121 tttaacgtca aggagaaaaa accccggatc cgtaaccatg tcaactggtt cttctcacca
      181 tcaccatcac catggttctt ctatgaaaat cgaagaaggt aaactggtaa tctggattaa
      241 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
      301 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
      361 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
      421 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
      481 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
      541 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
      601 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
      661 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
      721 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
      781 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
      841 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
      901 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
      961 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
     1021 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
     1081 ggttaataaa gacaaaccgc tgggtgccgt a
//
