LOCUS       sequence-7198-ex        4106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, 
            Bartel DP
  TITLE     The widespread impact of mammalian MicroRNAs on mRNA repression and 
            evolution.
  JOURNAL   Science. 2005 Dec 16. 310(5755):1817-21.
  PUBMED    16308420
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Dec 16. 310(5755):1817-21."
COMMENT     Sequence Label: pAG69 G6PD 3'UTR mut
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    421..542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(432..451)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     486..505
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        675..779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(780..848,849..1640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(998..1017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1811..2399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2300..2319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2528..2885
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2528..2719
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2551..2571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2736..2871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2955..3087
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3070..3089
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3132..3150
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3160..4095
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 cccccgagtc gggaggactc cgggaccatt gacctcagct gcagaatgct ggccccgggc
       61 tctggccacc ctggcccgcc cctcgctgct gctactaccc gagcccagct acattcctca
      121 gctgccaagc actcgagacc atcctggccc ctccagaccc tgcctgagcc caggagctga
      181 gtcacctcct ccactcactc cagcccaaca gaaggaagga ggagggcgcc cattcgtctg
      241 tcccagagct tattggccac tgggtctcac tcctgagtgg ggccagggtg ggagggaggg
      301 acaaggggga ggaaaggggc gagcacccac gtgagagaat ctgcctgtgg ccttgcccgc
      361 cagcctcagt gccacttgag aatgcttgtc atctagagcg gccgcttcga gcagacatga
      421 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      481 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      541 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      601 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt
      661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1021 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1321 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1921 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
     2461 ctcgacagat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     2521 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2641 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2701 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2761 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2821 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2881 gcaaaaagct tgattcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
     2941 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     3001 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     3061 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     3121 gctagagtac ttaatacgac tcactatagg ctagccacca tgacttcgaa agtttatgat
     3181 ccagaacaaa ggaaacggat gataactggt ccgcagtggt gggccagatg taaacaaatg
     3241 aatgttcttg attcatttat taattattat gattcagaaa aacatgcaga aaatgctgtt
     3301 atttttttac atggtaacgc ggcctcttct tatttatggc gacatgttgt gccacatatt
     3361 gagccagtag cgcggtgtat tataccagac cttattggta tgggcaaatc aggcaaatct
     3421 ggtaatggtt cttataggtt acttgatcat tacaaatatc ttactgcatg gtttgaactt
     3481 cttaatttac caaagaagat catttttgtc ggccatgatt ggggtgcttg tttggcattt
     3541 cattatagct atgagcatca agataagatc aaagcaatag ttcacgctga aagtgtagta
     3601 gatgtgattg aatcatggga tgaatggcct gatattgaag aagatattgc gttgatcaaa
     3661 tctgaagaag gagaaaaaat ggttttggag aataacttct tcgtggaaac catgttgcca
     3721 tcaaaaatca tgagaaagtt agaaccagaa gaatttgcag catatcttga accattcaaa
     3781 gagaaaggtg aagttcgtcg tccaacatta tcatggcctc gtgaaatccc gttagtaaaa
     3841 ggtggtaaac ctgacgttgt acaaattgtt aggaattata atgcttatct acgtgcaagt
     3901 gatgatttac caaaaatgtt tattgaatcg gacccaggat tcttttccaa tgctattgtt
     3961 gaaggtgcca agaagtttcc taatactgaa tttgtcaaag taaaaggtct tcatttttcg
     4021 caagaagatg cacctgatga aatgggaaaa tatatcaaat cgttcgttga gcgagttctc
     4081 aaaaatgaac aataattcta ggagct
//
